Numerous studies have demonstrated the association between aberrant methylation changes of the SHOX2/RASSFIA genes and cancer diagnosis.[22,23] The Methylated Human SHOX2 and RASSF1A Gene Detection Kit (Tellgen Co. Ltd., Shanghai, China) has been utilized for early lung cancer diagnosis and differentiation of benign and malignant pulmonary nodules.[24,25] Moreover, this kit can be employed to detect a variety of sample types, including BALF, tissue, pleural fluid, sputum, etc. In this study, we collected BALF samples from 36 patients with early LUAD and assessed their methylation status. Here, SHOX2 is linked to cancer.