REM2 and coinfection: In addition, GFP signal and anti-Rem2 staining were detected in the majority of neurons in CA1 in the animals that were injected with the virus expressing either wt-Rem2 or Rem2RR/GG and Cre-GFP (Fig 2A–2C) (Rem2 cKO + wt-Rem2, 70.0 ± 1.0%, 130 ± 11; Rem2 cKO + Rem2RR/GG, 87.5 ± 0.5%, 182 ± 17), demonstrating co-infection of the viruses in a high percentage of neurons in the targeted area.