We also performed Fluorescent Gelatin Degradation Assays for investigating invadopodia formation and found MFGE8-L up-regulation suppressed breast cancer cells invasion through a layer of extracellular matrix, whereas breast cancer cells with ectopic expression of MFGE8-S acquired enhanced ability to degrade matrix and invasion (Figure 5B). The gene discussed is MFGE8; the disease is breast cancer.