We utilized transwell chamber with or without matrix coat and would healing assays to examine the potential function of RBM7 in migration and invasion capabilities and found the depletion of RBM7 led to a visible increase in mobility and invasiveness of both the triple-negative breast cancer (TNBC) cell line MDA-MB-231, estrogen receptor positive breast cancer cell line MCF7 and 4T1 cells (Figure 2G–H and Figure 2—figure supplement 1B-E). This evidence concerns the gene ESR1 and breast carcinoma.