Besides, the relative expression of immune checkpoints in the three AML molecular subtypes were evaluated and the data suggested that nearly all the immune checkpoints including LAG3, PDCD1, PDCD1LG2, CD274, CTLA4, HAVCR2 and SIGLEC15, had remarkable difference in the three AML molecular subtypes (Fig. 8A). This evidence concerns the gene LAG3 and acute myeloid leukemia.