Besides, the relative expression of immune checkpoints in the three AML molecular subtypes were evaluated and the data suggested that nearly all the immune checkpoints including LAG3, PDCD1, PDCD1LG2, CD274, CTLA4, HAVCR2 and SIGLEC15, had remarkable difference in the three AML molecular subtypes (Fig. 8A). The gene discussed is PDCD1; the disease is acute myeloid leukemia.