To validate whether pSTAT1 binds to the mouse irf2 promoter, we treated RAW264.7 cells with recombinant IFNβ and treated CT26 tumor-bearing mice with IFNBCOL01, then performed ChIP analysis using a pSTAT1 specific antibody and PCR primers that covered the promoters approximately from −2000 to +2000 relative to the transcription start site of the mouse irf2 (Figure 6B). Here, IRF2 is linked to neoplasm.