While this was a limitation of the current investigation, analyzing the impact of Alisertib on PD-L1 expression in this glioblastoma model and further assessing the effects of AURKA inhibition in combination with PD-1/PD-L1 blockade is an important next step to more comprehensively elucidate the complex relationship between these critical signaling pathways in the context of this highly aggressive brain cancer. The gene discussed is PDCD1; the disease is brain cancer.