Engrailed Homeobox 2 gene (EN2) that, similar to RELN, controls pattern formation and dopaminergic neurogenesis during the development of the central nervous system and is linked to neuronal connectivity [36], trended for reduced expression in the NSCs and astrocytes of patients with autism, but in neurons exhibited increased expression (>3-fold, p = 0.05). This evidence concerns the gene RELN and autism.