Cross-sectional baseline quantitation of proteins included tTau, pTau181, Aβ42, Aβ40, NFL, and GFAP, while longitudinal neuropsychological measures comprised Mini-Mental State Examination (MMSE) and Consortium to Establish a Registry for Alzheimer’s Disease Assessment Packet (CERAD-TS) scores. Here, NEFL is linked to early-onset autosomal dominant Alzheimer disease.