Sk-Mel-28 BRAFi-R cells (SOX10 undetectable) were found to be highly resistant to A-485 effects on tumor cell growth following both short-term (6-day) and long-term (12-day) time points (Fig. 5D); however, the BRAFi-sensitive parental Sk-Mel-28 BRAFi-S (SOX10+) cells were sensitive to A-485 growth inhibition following long-term (12-day) treatment (Fig. 5B), suggesting that complete loss of SOX10 in Sk-Mel-28 BRAFi-R cells rendered them insensitive to A-485. This evidence concerns the gene SOX10 and neoplasm.