Furthermore, despite numerous attempts to confirm p300-mediated acetylation of SOX10 using Western blotting for acetyllysine and mass spectrometry of cell lysates from IPC-298 melanoma cells treated with DMSO, A-485, MG-132, or MG-132 + A-485, we were unable to confirm the precise mechanism of action governing p300 KAT–associated stabilization of SOX10 and suspect that this was due to the limitations of reagents used to detect lysine acetylation on Western blots and insufficient quantities of purified SOX10 protein for mass spectrometry analysis. The gene discussed is EP300; the disease is melanoma.