However, low detectability, complex glycan structures,and heterogeneity present challenges in glycoprotein assay development.Using haptoglobin (Hp) as a model, we developed an integrated platformcombining functionalized magnetic nanoparticles and zwitterionic hydrophilicinteraction liquid chromatography (ZIC-HILIC) for highly specificglycopeptide enrichment, followed by a data-independent acquisition(DIA) strategy to establish a deep cancer-specific Hp-glycosylationprofile in hepatitis B virus (HBV, n = 5) and hepatocellularcarcinoma (HCC, n = 5) patients. Here, HP is linked to hepatocellular carcinoma.