In summary, by integratingmagnetic nanoparticles and ZIC-HILICStage-Tips for highly specific enrichment of Hp-glycopeptide, we developedan integrated library-based DIA-MS platform that portrays the aberrationsin site-specific N-glycopeptides of haptoglobin in patient serum amongHBV and HCC disease groups. This evidence concerns the gene HP and hepatocellular carcinoma.