A3AR agonists induce dysregulation of Wnt and NF-kB signaling pathways, leading to apoptosis in HCC cells.Tumor cell proliferation is reduced, with decreased accumulation of G1 phase cells and inhibition of DNA and RNA synthesis.Reduced protein expression levels of β-conjugated proteins, patched1 (Ptch1), and glioma-associated oncogene homologous zinc finger protein 1 (Gli1) were found.The protein expression levels of cell cycle protein D1 and c-Myc were reduced. The gene discussed is ADORA3; the disease is hepatocellular carcinoma.