Moreover, through RNA sequencing analysis in tumor CD8+ T cells, we found that genes associated with effector functions, such as Sox6, Ccnd1, CD81 and Trim14, were upregulated, while genes associated with immune inhibition, such as Tox, Jun-b, Btla, Batf and Foxp1 were downregulated (Figure 1J) 25-27. This evidence concerns the gene CCND1 and neoplasm.