In comparison to factors such as age below 30, Unmarried, Unemployed, other Infection route, and subtypes like CRF07_BC and CRF08_BC, recent infection, and Pre-treatment Viral load <10000 copies/ml, factors associated with a higher risk for pre-treatment CD4+T lymphocyte decline included age over 30, divorced/widowed, peasant, heterosexual infection, CRF01_AE subtype, long-term infection, and pre-treatment viral load >10000 copies/ml. The gene discussed is CD4; the disease is infection.