Compared to single and double combined treatment, triple combined treatment with HFRT at a dose of 24 Gy in 3 fractions delivered to the primary tumor, LDRT at a dose of 2 Gy in 1 fraction delivered to the abscopal tumor, and systematic treatment with anti-mouse PD1 resulted in the greatest tumor response and longest survival, with most robust infiltration of CD8+ effector T cells and the lowest infiltration of myeloid-derived suppressor cells (MDSCs) in the abscopal tumor (23). Here, CD8A is linked to neoplasm.