In this study, IP-MS analysis indicated binding between RALY and OGT in Hep3B and BEL7402 cells (Figure 2A and Table S1) 18, indicating that RALY may be modified by O-GlcNAcylation, which was further confirmed through CO-IP assays and immunostaining in HCC cells (Figure 2B and 2C). This evidence concerns the gene RALY and hepatocellular carcinoma.