As shown in Fig. 3A and B, expression of the SHIP1-specific, but not of the control shRNA resulted in strong reduction of SHIP1 protein expression in both ALL cell lines and a concomitant increase in the phosphorylation of AKT by a factor of 45 (Reh) and 16 (SupB15), respectively. Here, AKT1 is linked to acute lymphoblastic leukemia.