To determine the expression level of CFAP65 in colon cancer and normal tissue, we detected CFAP65 expression in 20 pairs of frozen samples using both immunoblotting (Fig. 3a-c) and RT-qPCR (Fig. 3d-e), finding that CFAP65 displayed a higher expression in tumors compared to their matched paratumor tissue (Fig. 3c, e). The gene discussed is CFAP65; the disease is malignant colon neoplasm.