Two alveolar rhabdomyosarcoma (aRMS) lines, Rh41 and Rh3, which are t(2;13) PAX3-FOXO1 fusion-positive and express high levels of RUNX1T1, demonstrated significantly reduced cell proliferation following shRNA knockdown (Fig. 8d). The gene discussed is FOXO1; the disease is rhabdomyosarcoma.