DUSP18 and neoplasm: Since Dusp18 mediates tumor immune evasion in a CD8+ T cell-dependent manner, the cytotoxic killing of B16-OVA-Luc and MC38-OVA-Luc cells (expressing Ovalbumin and Luciferase) by OT-I T cells was measured in an in vitro killing system (Fig. 4e).