After incubation with activated CD8+ T cells, the cytotoxicity was measured by LDH release assays, and the results indicated that CD8+ T cells exerted lower cytotoxicity activity upon co-cultured with PD-L1 silencing (si-PD-L1) transfected NSCLC cells (H1299 cells) (Fig. 6F). The gene discussed is CD8A; the disease is non-small cell lung carcinoma.