PRNP and infection: Transposon insertion in the genes ptsP, prpC, and kinB led to marked suppression of the attenuation of Orsay virus leading to infection of 97%, 89%, and 82% of the population respectively compared to infection in only 4.2% of the population in the presence of wild-type P. aeruginosa PA14 (Figure 5B, Figure 5—figure supplement 1).