IGHE and allergic disease: Many diagnostic methods are used when investigating legume allergies, like in vivo tests (SPT, oral food challenge), functional tests in vitro and ex vivo (BAT, mast cell activation test (MAT), stripped basophil histamine release assay (BHRA), histamine release assay (HR) and lymphoproliferation assay (LPA)), in vitro non-functional tests (specific IgE, total IgE, IgE-immunoblotting, cross-reactive carbohydrate determinant (CCD)-IgE) and in silico (BLAST).