To evaluate the impact of ALS-associated variants on Annexin A11 localization and behaviour, we mosaically expressed eGFP-tagged wild-type, G38R and D40G Annexin A11 in motor neurons (injection of mnx1:gal4; UAS:AnxA11-GFP at the one-cell stage). This evidence concerns the gene MNX1 and amyotrophic lateral sclerosis.