MIF and aortic stenosis: To further highlight the importance of MIF-regulated signaling in patients with FP-AS, MIF was added to the network analysis displaying its interactions with significantly enriched gene ontology (Figure 3F) and Kyoto Encyclopedia of Genes and Genomes pathways (Figure S14) This observation is further substantiated by immunohistochemistry of AV tissue showing significantly increased MIF protein expression in FP-AS (Figure 3G).