The last two studies were CS by Wang et al. and Soares et al., one with a sample size of six patients (six eyes) and the other 21 patients (21 eyes), that evaluated the use of insulin 1 IU/mL two to four times daily for refractory corneal ulcers for a duration of 25 and 45 days, respectively [35,44]. The gene discussed is INS; the disease is corneal ulcer.