There were no statistically significant differences in the cycle type, infertility type, duration of infertility, age, BMI, basal FSH, basal LH, FSH/LH, basal P, basal E2, AFC, AMH, and the formation of high-quality cleavage embryos between the training set and the validation set (P > 0.05). This evidence concerns the gene PLOD1 and Infertility.