While the pathway terms enriched by down-regulated DEMs were biosynthesis of unsaturated fatty acids, prostate cancer, endocrine resistance, GnRH secretion, ovarian steroidogenesis, valine, leucine and isoleucine biosynthesis, pathways in cancer, valine, leucine and isoleucine degradation, aminoacyl-tRNA biosynthesis, nicotinate and nicotinamide metabolism and steroid hormone biosynthesis (Fig. 4C). Here, GNRH1 is linked to Familial prostate cancer.