In this study, we explored the activation of these pathways in freshly isolated breast tumors resulting from the overexpression of wild-type RRAS2. We analyzed the phosphorylation levels of key components of the PI3K/Akt, Raf-ERK, and mTORC1 pathways using western blotting in breast tumors from four Rosa26-RRAS2fl/fl x Wap-Cre breeder female mice and compared them with paired non-tumoral mammary glands from the same mice. Here, PIK3CA is linked to breast neoplasm.