Key driver analyses revealed the most influential network node to be an antisense transcript AC131944.1 (ENSG00000286618.1, Fig. 4A), which, to our knowledge, has not been described in the context of microglial biology or AD, but which is encoded antisense to SPP1. In addition, we observed APOE as a key network driver in CD83(+) microglia, whereas we did not observe it as a network driver in non-CD83(+) microglia using a permutation-based network comparison approach (Fig. 4C). Here, SPP1 is linked to Alzheimer disease.