Cryo-FM of these vitrified postmortem AD tissues revealed the location of MX04-labelled amyloid pathology, including neuritic plaques (Fig. 1e), tau tangles (Fig. 1f) and threads (Fig. 1e,f), which resembled those in fixed tissue (Fig. 1c and Extended Data Fig. 2b). This evidence concerns the gene MAPT and Alzheimer disease.