Here, we performed editing by nonhomologous DNA end joining (NHEJ) to target a dominant Mir96 mutation (Mir96tm3.1Wtsi, referred to as Mir9614C>A in this report; equivalent of human rs587776523, g.7:129414596G>T in GRCh38) by AAV-mediated delivery in young adult mice with hearing loss. The gene discussed is MIR96; the disease is hearing loss disorder.