To identify potential pathways related to Tks4 and EMT, we used a Tks4 immunoprecipitation (IP)-mass spectrometry (MS) method to identify novel Tks4 interactors and analyzed the Tks4-associated signaling proteins and their links to EMT regulation in three different lung cancer cell lines (A549, HOP-92, NCI-H460). This evidence concerns the gene SH3PXD2B and lung cancer.