detected the expression of six kind of cell surface receptors, including PD-L1, B7 -H3, B7-H4 and VISTA which could be targeted by CAR-T in forty-five skull base chordoma samples, the results showed that B7-H3 had the highest positive rate (7/45, 16%) in samples, and the positive rate was ranked as B7-H3 > PD-L1 > VISTA = B7-H4, suggesting that B7-H3 was a good target for CAR-T treatment of chordoma (45). The gene discussed is CD274; the disease is chordoma.