To assess whether NDRG1 is responsible for the anti-cancer effect of the mitochondrially targeted iron chelators and to compare their biological activity, we generated NDRG1 knockout (KO) and overexpressing (OE) clones (CCDS 34945, CCDS59112 and 59,113) in the MCF7 and MDA-MB-231 cells. This evidence concerns the gene NDRG1 and cancer.