Therefore, to investigate the potency of different netarsudil-family ROCKi compounds in rescuing pathologic HTM hydrogel contraction, constructs were treated with TGFβ2 for 5 d to induce a glaucoma-like cell phenotype before adding netarsudil/compounds A-D over a broad dose range for the next 5 d in the absence of TGFβ2. This evidence concerns the gene ROCK1 and glaucoma.