Therefore, the goal of the present study was to use our HTM hydrogel as screening platform to investigate the effects of clinically-used netarsudil and different netarsudil-family experimental ROCKi compounds on reversing TGFβ2-induced pathologic HTM cell contraction and actin stress fiber formation – both strongly associated with outflow dysfunction in glaucoma. The gene discussed is ROCK1; the disease is glaucoma.