In another study, Aparicio et al. compared four methods, MethyLight, COBRA, MS-SNuPE, and pyrosequencing, to analyze the methylation of the melanoma-associated antigen 1 (MAGE-A1) and LINE-1 promoters in HCT116 (colon cancer cells) and T24 (bladder cancer cells). This evidence concerns the gene MAGEA1 and colonic neoplasm.