In the present study, the inhibitory effects of DHBA/GPR81 on the anti-fibrotic CREB/Smad7 pathway have been verified in TGF-β1-activated LX-2 cells and CCl4-insulted mice, which might represent an important mechanism underlying the detrimental activity of GPR81 in liver fibrosis. The gene discussed is TGFB1; the disease is Hepatic fibrosis.