To determine whether tumor cell-intrinsic IFN signaling pathways might contribute to the therapeutic effect of CV-1, IFN-I receptor (IFNAR1)-knockout (KO) and IFN-II receptor (IFNGR1) KO MC38 cell lines were constructed using CRISPR-Cas9 (Supplementary Fig. 1a, b). This evidence concerns the gene IFNAR1 and neoplasm.