To investigate whether ZBP1 induces necroptosis and inflammation via Zαβ-dependent sensing of Ζ-ΝΑ ligands and RHIM-mediated signaling, we assessed the condensate formation and downstream necrosome formation in response to viral infection using HT-29 cells expressed inducible GFP-tagged wild-type and mutant ZBP1 (Fig. S5A). Here, ZBP1 is linked to viral infectious disease.