Growing evidence indicates DCLK1 overexpression within the CCA cancer stem cells (CSCs) subpopulation and in serum samples from patients with CCA, and might be a potential predictive biomarker for early diagnose in CCA(Nevi et al. 2021; Meadows and Francis 2021; Andresen et al. 2015). The gene discussed is DCLK1; the disease is cholangiocarcinoma.