Furthermore, qPCR analysis revealed markedly elevated expression levels of inflammatory factors, including IL-6, TNF-α, IL-1β, CCL2 and CXCL10, in the PBMCs, lungs, skeletal muscles, spinal cord and pancreas tissues of the severe HFMD mice compared to the control group (Figures 2E–I). Here, IL6 is linked to hand, foot and mouth disease.