NFKB1 and cancer: Figure 2F demonstrated that the KEGG analysis of increased DEGs in the old eye cup was predominantly enriched in cytokine-cytokine receptor interaction, complement and coagulation cascades, phagosome, NK cell mediated cytotoxicity, Th1 and Th2 cell differentiation, IL17, JAK-STAT and NF-κB signaling pathway. The main pathways involving down-regulated DEGs included ECM–receptor interaction, calcium signal pathway, proteoglycans in cancer, as well as protein digestion and absorption (Figure 2F).