CHAT and Alzheimer disease: The colocalization of hTau and ChAT at BF was evaluated in young and old AD mice by Pearson’s correlation coefficient (Rp) on ROI, and the average Rp assessed on multiple ROI within BF was higher in young versus in old 3xTgAD mice (Fig. 4I): P = 0.004, unpaired 2-tailed t-test with equal variance, t = 3.205, df = 27, n = 3–5 mice/group.