MAPT and Alzheimer disease: ChAT+ cells located within the BF near and below the CPu of striatum in representative images on sagittal sections of 3xTgAD mice (Fig. 3A and D), and pathological tau protein appeared as early as 4–6 months of age within the BF of 3xTgAD mice (Fig. 3C; Supplementary Fig. 2) prior to the appearance of extracellular Aβ plaques in the BF of AD mice (Fig. 3F; Supplementary Fig. 3).