To validate the essential roles of FOXM1 in CDCA5 induced proliferation and migration of breast cancer cells, functional rescue experiments in BT-549 and MDA-MB-231 cells were performed by transfection of CDCA5 lentivirus, shFOXM1 lentivirus and co-transfection of CDCA5 and shFOXM1 lentivirus. This evidence concerns the gene CDCA5 and breast cancer.