Univariate analysis revealed that 10 out of the 16 potential influencing factors exhibited statistical significance on the outcome of live birth: age (P < 0.001), OSI (P = 0.003), infertility cause (P = 0.048), COS treatment regimen (P < 0.001), Gn starting dose (P = 0.001), endometrial thickness on HCG day (P = 0.007), LH value on HCG day (P < 0.001), P value on HCG day (P = 0.032), 2PN number (P = 0.049) and embryo transfer strategy (P < 0.001). The gene discussed is PLOD1; the disease is Infertility.