Using different human monocyte isolation kits and “untouched” monocytes from patients with primary immune thrombocytopenia (ITP), an autoimmune disease characterized by low blood platelet counts, and in vivo platelet depletion in mice, we demonstrate that platelet numbers directly impact the cytokine output of CD14+ monocytes towards Toll-like receptor (TLR) and Nod-like receptor (NLR) stimulation. Here, CD14 is linked to autoimmune thrombocytopenic purpura.