The unpaired student’s t-test showed the decrease in protein expression of ANK2, SYNE1 and NLGN3 was significant in the cancer cell lines MDA-MB-231 (p-value < 0.05) when compared with control MCF10A and decrease in protein expression of SYNE1 was significant in MCF7 breast cancer cell line (Fig. 4h–j). The gene discussed is ANK2; the disease is cancer.