The distribution of tumor budding in categories evaluated by H&E was as follows: 115 (23%) Bd0, 217 (44%) Bd1, 108 (22%) Bd2, and 53 (11%) Bd3, whereas assessment by IHC resulted in 21 (4%) Bd0, 104 (21%) Bd1, 111 (23%) Bd2, and 257 (52%) Bd3. Here, DEFB1 is linked to neoplasm.