PRNP and infection: It is notable that upon i.p. infection, prions with identical conformational stability, and similar distribution and vacuolation profiles emerged from deer CWD isolates that, when inoculated i.c. into deer PrPC overexpressing mice propagated as distinct and stable strains and substrains, Wisc-1/CWD1, and 116AG (short and long), respectively [25,26,28,29], with no evidence of common strain components [29].