REEP5 and tuberculosis: Our results show that the QFT-CLIA platform reports consistently higher IFN-γ values than QFT-ELISA for both TB1 and TB2, translating into a 16.6% higher positivity rate for QFT-CLIA within this cohort of TB contacts, with most of the samples being within the borderline range of positivity (0.35–0.99 IU/mL).